Published

Microelectronics

High-throughput single-cell electroporation microchip with three dimensional si microelectrodes for gene transfection

Published on - Transducers '07 & Eurosensors Xxi, Digest of Technical Papers, Vols 1 and 2

Authors: Y. H. Cho, S. W. Lee, Bruno Le Pioufle, N. Takama, S. Takeuchi, T. Fujii, B. J. Kim

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We present details on fabrication of electroporation microchip, practical experiments of single-cell electroporation with our fabricated microchip. Also, the continuous electroporation for the continuous flow of cells is used for high-throughput electroporation. The delivery efficiency and cell viability tests are provided and the successful GFP transfection into cells is also evaluated with a fluorescent microscope after electroporation.