Electric power

A silicon micro-system for parallel gene transfection into arrayed cells

Publié le - 8th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Auteurs : A. Tixier-Mita, J. Jun, M. Chiral, S. Ostrovidov, Marie Frénéa-Robin, Bruno Le Pioufle, H. Fujita

We successfully made a silicon bio-micro-system able to capture several hundreds of cells as a regular array and to place each cell between microelectrodes for electroporation. We confirmed that some fluorescent dye was successfully inserted by electroporation in 75% of living captured cells; this is the first step towards parallel processing of gene insertion to individual cells.