Reproducing topography and roughness of osteoconductive materials in a microfluidic device for bone tissue engineering

This paper is in the framework of tissue engineering, based on bioreactor for cultivating bone-forming cells on biomimetic materials, such as coral scaffold. In order to optimize such process and to have a better understanding of biophysical and biochemical mechanisms involved in osteoblast proliferation, we develop a thermoplastic microfluidic chip for cell growth studies at the cell scale. The surface porosity (100 μm), roughness (aragonite structure