Out-of-Equilibrium Measurements of Kinetic Constants on a Biosensor

Conventional measurements of kinetic constants currently in use are performed at equilibrium and may require large volumes, especially at low association rate constant k on. If the measurements are made out of equilibrium, the values obtained may be biased by dilution of the sample with the flow of the running buffer. In some applications, the available sample volume can be very critical and requires the developmentof tools to measure kinetic constants with low volumes. In this paper, through ex-perimental, numerical simulation and a modeling combined approach, we propose aSPR-based method that relies on an out-of-equilibrium measurement, using the effectof dilution by flow to its advantage. This new method should have a significant impactin biochemistry and medical research