Microfluidic device using reusable parylen-pdms packaging for the red blood cell transit time analysis in mechanical constrictions, using impedance measurement

In this paper, a micro-device dedicated to the analysis of the Red Blood Cell (RBC) deformability is proposed. It is based on PDMS microfluidic channels mimicking the flowing in the capillary vascular network. The RBC transit time within the capillary is electrically sensed using embedded electrodes on glass associated to current blockage analysis. To get reusable device, a specific PDMS coated by parylen has been developed. In this way, the PDMS porosity to gaz is drastically reduced which permits to used depressurization for both controlling the biological sample flow and the non-permanent bonding of the device for reusable capability.