Conception et réalisation d'un bio-microsystème basé sur la diélectrophorèse et l'électrofusion en vue de l'immunothérapie du cancer

Our subject of research comes from a request of clinicians to obtain in great quantity the hybridomas resulting from fusion between cancer cells and dendritic cells. This work forms part of research against cancer by a process close to vaccination, called immunotherapy of cancer. There exist currently marketed devices, but those do not manage to produce in an important way hybridomas of qualities. The idea was to place itself at the level of the cells, to interact on them in a precise way, while profiting from the miniaturization to control a large number of cells by paralleling the device. Remained to choose which forces to use to act on the cells to move them, to place them and to fusion them, because on a microscopic scale the report of the forces are different from those on our scale. We chose the use of microfluidic for the transport of the cells, the dielectrophoresis to position them and the electrofusion to fusion them. This project is with the conjunction of know-how in the fields of the microtechnology, the interaction field electric-cells and cellular biology.